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nonimmune rabbit serum (10  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology nonimmune rabbit serum (10
    Nonimmune Rabbit Serum (10, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nonimmune+rabbit+serum+(10/ImmunoCruz+rabbit+ABC+Staining+System/pmc06881566-57-13-16
    Average 90 stars, based on 1 article reviews
    nonimmune rabbit serum (10 - by Bioz Stars, 2026-10
    90/100 stars

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    Incubation:

    Article Title: Channel-dependent permeation of water and glycerol in mouse morulae.
    Article Snippet: .. Then they were incubated with blocking solution; PBS containing 10% nonimmune donkey serum (Santa Cruz Biotechnology, Inc.) and 5 mg/ml bovine serum albumin (for AQP3) or 10% nonimmune goat serum (Santa Cruz Biotechnology, Inc.) and 5 mg/ml bovine serum albumin (for AQP7) at 258C for 60 min. After being rinsed, they were incubated with diluted anti-human AQP3 goat antibody (1/400) or anti-mouse AQP7 rabbit antiserum with blocking solution at 258C for 60 min. As a control, instead of the primary antibodies, anti-human AQP3 goat antibody or anti-mouse AQP7 rabbit antiserum preincubated with a blocking peptide, which was the same peptide used for an antigen, at room temperature for 1 h, was used for the experiments. .. After being rinsed, the oocytes and embryos were incubated with diluted fluorescein isothiocyanate (FITC)-conjugated anti-goat IgG donkey antibody (1/1600) (Santa Cruz Biotechnology, Inc.) (for AQP3) or diluted FITCconjugated anti-rabbit IgG goat antibody (Chemicon International, Temecula, CA) (for AQP7) with blocking solution at 258C for 30 min.

    Blocking Assay:

    Article Title: Channel-dependent permeation of water and glycerol in mouse morulae.
    Article Snippet: .. Then they were incubated with blocking solution; PBS containing 10% nonimmune donkey serum (Santa Cruz Biotechnology, Inc.) and 5 mg/ml bovine serum albumin (for AQP3) or 10% nonimmune goat serum (Santa Cruz Biotechnology, Inc.) and 5 mg/ml bovine serum albumin (for AQP7) at 258C for 60 min. After being rinsed, they were incubated with diluted anti-human AQP3 goat antibody (1/400) or anti-mouse AQP7 rabbit antiserum with blocking solution at 258C for 60 min. As a control, instead of the primary antibodies, anti-human AQP3 goat antibody or anti-mouse AQP7 rabbit antiserum preincubated with a blocking peptide, which was the same peptide used for an antigen, at room temperature for 1 h, was used for the experiments. .. After being rinsed, the oocytes and embryos were incubated with diluted fluorescein isothiocyanate (FITC)-conjugated anti-goat IgG donkey antibody (1/1600) (Santa Cruz Biotechnology, Inc.) (for AQP3) or diluted FITCconjugated anti-rabbit IgG goat antibody (Chemicon International, Temecula, CA) (for AQP7) with blocking solution at 258C for 30 min.

    Control:

    Article Title: Channel-dependent permeation of water and glycerol in mouse morulae.
    Article Snippet: .. Then they were incubated with blocking solution; PBS containing 10% nonimmune donkey serum (Santa Cruz Biotechnology, Inc.) and 5 mg/ml bovine serum albumin (for AQP3) or 10% nonimmune goat serum (Santa Cruz Biotechnology, Inc.) and 5 mg/ml bovine serum albumin (for AQP7) at 258C for 60 min. After being rinsed, they were incubated with diluted anti-human AQP3 goat antibody (1/400) or anti-mouse AQP7 rabbit antiserum with blocking solution at 258C for 60 min. As a control, instead of the primary antibodies, anti-human AQP3 goat antibody or anti-mouse AQP7 rabbit antiserum preincubated with a blocking peptide, which was the same peptide used for an antigen, at room temperature for 1 h, was used for the experiments. .. After being rinsed, the oocytes and embryos were incubated with diluted fluorescein isothiocyanate (FITC)-conjugated anti-goat IgG donkey antibody (1/1600) (Santa Cruz Biotechnology, Inc.) (for AQP3) or diluted FITCconjugated anti-rabbit IgG goat antibody (Chemicon International, Temecula, CA) (for AQP7) with blocking solution at 258C for 30 min.

    Staining:

    Article Title: Enhanced Endothelin A and B Receptor Expression and Receptor-Mediated Vasoconstriction in Rat Mesenteric arteries after Lipopolysaccharide Challenge
    Article Snippet: Sections were then visualized with a DAB kit (Maixin Biotech, Fuzhou, China), counterstained with hematoxylin, and observed under an Echo Revolve hybrid microscope (Echo Laboratories, San Diego, CA, USA). .. No detectable staining was observed in all negative control slides treated with nonimmune rabbit serum (10%) (Santa Cruz Biotechnology, Santa Cruz, CA, USA). ..

    Article Title: Enhanced Endothelin A and B Receptor Expression and Receptor-Mediated Vasoconstriction in Rat Mesenteric arteries after Lipopolysaccharide Challenge.
    Article Snippet: Sections were then visualized with a DAB kit (Maixin Biotech, Fuzhou, China), counterstained with hematoxylin, and observed under an Echo Revolve hybrid microscope (Echo Laboratories, San Diego, CA, USA). .. No detectable staining was observed in all negative control slides treated with nonimmune rabbit serum (10%) (Santa Cruz Biotechnology, Santa Cruz, CA, USA). ..

    Negative Control:

    Article Title: Enhanced Endothelin A and B Receptor Expression and Receptor-Mediated Vasoconstriction in Rat Mesenteric arteries after Lipopolysaccharide Challenge
    Article Snippet: Sections were then visualized with a DAB kit (Maixin Biotech, Fuzhou, China), counterstained with hematoxylin, and observed under an Echo Revolve hybrid microscope (Echo Laboratories, San Diego, CA, USA). .. No detectable staining was observed in all negative control slides treated with nonimmune rabbit serum (10%) (Santa Cruz Biotechnology, Santa Cruz, CA, USA). ..

    Article Title: Enhanced Endothelin A and B Receptor Expression and Receptor-Mediated Vasoconstriction in Rat Mesenteric arteries after Lipopolysaccharide Challenge.
    Article Snippet: Sections were then visualized with a DAB kit (Maixin Biotech, Fuzhou, China), counterstained with hematoxylin, and observed under an Echo Revolve hybrid microscope (Echo Laboratories, San Diego, CA, USA). .. No detectable staining was observed in all negative control slides treated with nonimmune rabbit serum (10%) (Santa Cruz Biotechnology, Santa Cruz, CA, USA). ..



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